he non-toxic and uniformly sized anionic nanoparticles (NPs) with consistent size, composition, and negative surface charge that can be loaded with traditional water-soluble synthetic drugs and, alternatively, protein therapeutics in the lumen and on the surface of the vesicles. In this uniform single nanosize DNA nanoparticle, the negatively charged backbones of ssDNA or RNA will bind water soluble synthetic drugs or proteins through electrostatic interaction. This product can be used for drug delivery. 

This DNA nanoparticle also can be used as building block for innovative nanostructures for science and engineering investigations.